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Tris acetate munyu inowanzoshandiswa mukugadzirira TAE (Tris-acetate-EDTA) buffer, iyo inoshandiswa sechinomhanya chinomhanya uye mune agarose gels.Tris Acetate-EDTA buffer inoshandiswa DNA agarose gel electrophoresis asi inoshandiswawo kune isiri-denaturing RNA agarose gel electrophoresis.DNA ine tambo mbiri inowanzomhanya nekukurumidza muTAE kupfuura mune mamwe mabuffers uye inogona kupera simba panguva yakawedzerwa electrophoresis.Buffer kutenderera kana kutsiva buffer panguva yakawedzerwa electrophoresis inogona kugadzirisa yakaderera buffering.Inogona kushandiswa munzvimbo dzakasiyana siyana kudzidza mafambiro eDNA mumhinduro.Sezvo borate muTBE buffer (Tris-Borate-EDTA Buffer, 10X Powder Pack, sc-296651) inhibitor yakasimba yemaenzayimu akawanda, TAE buffer inokurudzirwa kana uchitarisa enzymatic application yeDNA sample.Tris acetate munyu zvakare ibuffer ine high senitivity muATP assays ine firefly luciferase.

Zvishandiso: TAE inomhanya buffer ndiyo inonyanya kushandiswa buffer yeDNA agarose gel electrophoresis, uye inoshandiswawo kune yekuzvarwa RNA agarose gel electrophoresis.DNA ine tambo mbiri inoita kufamba nekukurumidza muTAE kupfuura mune mamwe mabuffers, asiwo inotadza kushambira nekuda kwekushaikwa kwebuffer ion panguva ye electrophoresis yakareba.Buffer cycling kana buffer exchange panguva yakareba electrophoresis inogona kubhadhara iyo yakaderera buffer kugona.2 Diraisa iyo yakanyura TAE buffer kuti uwane 1 mMTAE bhafa ine 40 mM Tris acetate uye 1 mM EDTA, pH 8.3.1 mMTAE buffer inogona kushandiswa ese ari agarose gels uye seanomhanya buffer.Pakugadziriswa kwepamusoro, zvinokurudzirwa kuti magetsi anoshandiswa ave akaderera pane 5V / cm (kure pakati peyuniti electrodes).

Kushandisa: TAE inomhanya buffer ndiyo inonyanya kushandiswa buffer yeDNA agarose gel electrophoresis mu Chemicalbook gel, uye inoshandiswawo kune isiri-denaturing RNA agarose gel electrophoresis.DNA ine tambo mbiri inoita kufamba nekukurumidza muTAE kupfuura mune mamwe mabuffers, asiwo inotadza kushambira nekuda kwekushaikwa kwebuffer ion panguva ye electrophoresis yakareba.Buffer cycling kana buffer exchange panguva yakareba electrophoresis inogona kubhadhara iyo yakaderera buffer kugona.The concentrated TAE buffer yakadzikiswa kuti iwane 1 mMTAE buffer ine 40 mM Tris acetate uye 1 mM EDTA, pH 8.3.1 mMTAE buffer inogona kushandiswa ese ari agarose gels uye seanomhanya buffer.Pakugadziriswa kwepamusoro, zvinokurudzirwa kuti magetsi anoshandiswa ave akaderera pane 5V / cm (kure pakati peyuniti electrodes).

Zvishandiso: Pakuonekwa kweATP nefirefly luciferase, chigadzirwa ichi ndicho chinotyisa zvakanyanya;glutamate inosunga kuona.

Displaceable kusunga kwe [3H]l-glutamic acid kune zvisiri zvekugamuchira zvinhu.

[3H]L-glutamic acid inosunga kumachubhu emicrofuge negirazi yakaongororwa mumabhafa mana.Background inosunga kuzvinhu izvi yakanga isingakoshi, asi yakawedzerwa ne centrifugation kana kusveta muTris-HCl uye Tris-citrate buffer.Kusunga uku kwaive kushoma kana Pakashandiswa HEPES-KOH, kana Tris-acetate buffer panzvimbo.[3H]L-glutamate inosunga kune microfuge tubes yakavharwa neL-asi kwete D-isomers ye glutamate uye aspartate.DL-2-amino-7-phosphonoheptanoic acid zvakare haina kurambidza kusunga.Mimwe mishonga yakaratidza kuderera kusvika pakati-kati inhibition yaiva: N-methyl-D-aspartate, quisqualate, L-glutamic acid diethyl ester, N-methyl-L-aspartate, kainate, uye 2-amino-4 -phosphonobutyrate.Kusunga kwakavharwa nedenatured rat brain membranes.Iprotein-inotsamira [3H] glutamate inosunga yakawanikwa neyakadzokororwa-yakanyungudutswa membrane gadziriro apo kusunga kwaitwa muTris-acetate buffer.Zvinokurudzirwa kuti Tris-acetate kana HEPES -KOH buffer inofanira kushandiswa mu glutamate bin ding assay.Kana Tris-HCl kana Tris-citrate buffer ikashandiswa, kuyedza kutonga kwakakodzera kunofanirwa kuitwa kugadzirisa kusungirira kumachubhu emicrofuge kana mafirita egirazi.