X-GAL CAS:7240-90-6 98% Ichena kusvika kunze-chena Crystalline Powder
| Catalog Number | XD90008 |
| Product Name | X-Gal (5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) |
| CAS | 7240-90-6 |
| Molecular Formula | C14H15BrClNO6 |
| Molecular Weight | 408.63 |
| Storage Details | -2 kusvika -6 °C |
| Harmonised Tariff Code | 29400000 |
Chigadzirwa Specification
| Kuonekwa kweSolution | Yakajeka, isina ruvara kusvika kuchiedza yero mhinduro (50mg/ml muDMF:MeOH, 1:1) |
| Specific optical rotation | -61.5 +/- 1 |
| Chitarisiko | Ichena kusvika kunze-chena crystalline poda |
| Purity HPLC | zvishoma 99% |
| Solubility (5% muDMF) | Soluble (5% w/v,DMF) |
| Mvura KF | kudarika 1% |
| Assay (HPLC pane Anhydrous Basis) | min 98% w/w |
Kushandiswa kweX-gal
X-gal (zvakare yakapfupikiswa BCIG ye 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) is organic compound inosanganisira galactose yakabatanidzwa kune inotsiva indole.Mushonga wacho wakagadzirwa naJerome Horwitz uye vabatsiri muna 1964. Zita remakemikari rinowanzopfupikiswa kuti rive risinganyanyi kurongeka asiwo zvirevo zvisinganyanyi kunetsa senge bromochloroindoxyl galactoside.Iyo X kubva ku indoxyl inogona kunge iri sosi yeX mune X-gal contraction.X-gal inowanzoshandiswa mumolecular biology kuyedza kuvepo kweenzyme, β-galactosidase, panzvimbo yainotarirwa, β-galactoside.Inoshandiswawo kuona chiitiko cheiyo enzyme mu histochemistry uye bacteriology.X-gal ndeimwe yeakawanda indoxyl glycosides uye esters iyo inoburitsa isinganyunguke yebhuruu komputa yakafanana nedhayi yeindigo semhedzisiro ye enzyme-catalyzed hydrolysis.
X-gal ianalogi yelactose, uye nekudaro inogona kuve hydrolyzed ne β-galactosidase enzyme inotsemura β-glycosidic bond muD-lactose.X-gal, kana yatsemurwa ne β-galactosidase, inopa galactose uye 5-bromo- 4-chloro-3-hydroxyindole - 1. Iyo yekupedzisira inobva yangoerekana yaita dimerize uye inogadzirwa mu 5,5'-dibromo-4,4'-dichloro -indigo - 2, chigadzirwa chebhuruu chakanyanya chisinganyungudika.X-gal pachayo haina ruvara, saka kuvepo kweblue-ruvara chigadzirwa kunogona kushandiswa seyedzo yekuvapo kweanoshanda β-galactosidase.Izvi zvinobvumirawo kuti bhakitiriya β-galactosidase (inonzi lacZ) ishandiswe semutori wenhau mune zvakasiyana-siyana.
Mukuongorora-masanganiswa maviri, β-galactosidase inogona kushandiswa semutori wenhau kuona mapuroteni anodyidzana.Nenzira iyi, maraibhurari egenome anogona kuongororwa kupindirana kweprotein uchishandisa mbiriso kana bhakitiriya system.Ikoko kune kudyidzana kwakabudirira pakati pemapuroteni ari kuongororwa, zvinozoguma nekusungwa kweiyo activation domain kune anosimudzira.Kana mutsigiri akabatanidzwa nejene re lacZ, kugadzirwa kwe β-galactosidase, iyo inoguma nekuumbwa kwebhuruu-pigmented colonies pamberi pe X-gal, saka icharatidza kubudirira kwekubatana pakati pemapuroteni.Iyi nzira inogona kugumira pakuongorora maraibhurari esaizi isingasviki kutenderedza 106. Iyo inobudirira cleavage yeX-gal inoitawo kunhuhwirira kunooneka nekuda kwekuparara kweindole.
Sezvo X-gal pachayo isina ruvara, kuvapo kwechigadzirwa chebhuruu-ruvara kunogona kushandiswa seyedzo yekuvapo kweiyo inoshanda β-galactosidase.
Kuzivikanwa kuri nyore uku kweiyo enzyme inoshanda inobvumira gene re βgalactosidase (iyo lacZ gene) kuti ishandiswe sereporter gene mukushandisa kwakasiyana.
