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Sigma receptor 1 (σR1) isiri-opioid transmembrane protein inogona kuita se molecular chaperone pa endoplasmic reticulum-mitochondrial membrane.Ligands ye σR1, yakadai se (+)-pentazocine [(+)-PTZ], inopa yakanyorwa retinal neuroprotection mu vivo uye in vitro.Munguva pfupi yapfuura takaongorora retinal phenotype yemakonzo asina σR1 (σR1 KO) uye takaona yakajairika retinal morphology uye kushanda mumakonzo madiki (5-30 mavhiki) asi yakadzikira yakashata scotopic chikumbaridzo mhinduro (nSTRs), retinal ganglion cell (RGC) kurasikirwa, uye kukanganisa. ye optic nerve axons inoenderana nekusashanda kwemukati retinal ne1 gore.Iyi data yakaita kuti tiedze kufungidzira kuti σR1 inogona kunge yakakosha mukudzivirira kusingaperi retinal kusagadzikana;Chirwere cheshuga chaishandiswa semuenzaniso wekushushikana kusingaperi.Kuona kana σR1 ichidikanwa kune (+) -PTZ neuroprotective mhedzisiro, yekutanga RGCs yakaparadzaniswa nemhando-yemhando (WT) uye σR1 KO mbeva dzakaratidzwa xanthine-xanthine oxidase (10 µM: 2 mU/ml) kuita kuti oxidative kusagadzikana kuvepo kana kusavapo kwe (+) -PTZ.Kufa kwesero kwakaongororwa neterminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kuongororwa.Kuongorora mhedzisiro yekusagadzikana kusingaperi pane RGC basa, chirwere cheshuga chakakonzerwa mu3-vhiki C57BL/6 (WT) uye σR1 KO mbeva, vachishandisa streptozotocin kuburitsa mapoka mana: WT nondiabetic (WT isiri-DB), WT chirwere cheshuga (WT-DB). ), σR1 KO isiri-DB, uye σR1 KO-DB.Mushure memavhiki e12 echirwere cheshuga, apo mbeva dzaive nemavhiki e15, intraocular pressure (IOP) yakanyorwa, kuongororwa kwe electrophysiologic kwakaitwa (kusanganisira kuonekwa kwenSTRs), uye nhamba yeRGCs yakaverengwa mu retinal histological sections.In vitro zvidzidzo zvakaratidza kuti (+)-PTZ haina kukwanisa kudzivirira oxidative-induced death of RGCs yakakohwewa kubva kuR1 KO mbeva asi yakapa dziviriro yakasimba parufu rweRGCs dzakakohwewa kubva kuWT mbeva.Muzvidzidzo zvekushushikana kusingaperi kunokonzerwa nechirwere cheshuga, iyo IOP yakayerwa mumapoka mana emakonzo yaive mukati meyakajairika;zvisinei, pane kuwedzera kukuru kweIOP ye σR1 KO-DB mbeva (16 ± 0.5 mmH g) zvichienzaniswa nemamwe mapoka akaedzwa (σR1 KO isiri-DB, WT isiri-DB, WT-DB: ~12 ± 0.6 mmHg )Nezve electrophysiologic test, iyo nSTRs ye σR1 KO isiri-DB mice yakanga yakafanana neWT isiri-DB mice pamasvondo e15;zvisinei, vaive vakaderera zvakanyanya mu σR1 KO-DB mbeva (5 ± 1 µV) zvichienzaniswa nemamwe mapoka, kusanganisira, kunyanya, σR1 KO-nonDB (12±2 µV).Sezvaitarisirwa, nhamba yeRGCs mu σR1 KO isiri-DB mbeva yakanga yakafanana neWT isiri-DB mbeva pamavhiki e15, asi pasi pekunetseka kusingaperi kwechirwere cheshuga kwaiva neRGCs shoma mu retinas ye σR1 KO-DB mice.Iyi ndiyo rondedzero yekutanga. kuratidza zvisina mubvunzo kuti neuroprotective mhedzisiro ye (+) -PTZ inoda σR1.σR1 KO mbeva dzinoratidza zvakajairika retinal chimiro uye basa pazera diki;zvisinei, kana zvichiiswa mukushushikana kusingaperi kwechirwere cheshuga, kune kukurumidza kweretinal functional deficits mu σR1 KO mbeva zvekuti ganglion cell dysfunction inoonekwa pazera rekutanga kupfuura nondiabetic σR1 KO mbeva.Iyo data inotsigira pfungwa yekuti σR1 inobata basa rakakosha mu modu lating retinal kusagadzikana uye inogona kuve yakakosha chinangwa chechirwere che retinal.