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Isu takangotaura kuti pyrene nucleotide inonyanya kuiswa pakatarisana nenzvimbo yebasa, iyo 3'-T yethymine dimer, uye mabhesi mazhinji asina kukuvara nembiriso DNA polymerase eta (pol eta).Nekuda kwekuti pyrene is a nonpolar molecule isina H-bonding kugona, iyo isingawanzo kukwirira kushanda kwe dPMP yekuisa inoratidzwa kune yayo yepamusoro base stacking kugona, uye inosimbisa kukosha kweiyo base stacking mukusarudzwa kwema nucleotides nepol eta.Kuti tiongorore basa reH-bonding uye base pair geometry mukusarudzwa kwema nucleotides nepol eta, takasarudza kuiswa kwehutano hwe base-modified nucleotides 2,6-diaminopurine, 2-aminopurine, 6-chloropurine, uye inosine iyo inogona. ita nhamba yakasiyana yeH-bond ne template base zvichienderana nebhesi pair geometry.Watson-Crick base pairing inoratidzika kuita basa rakakosha pakusarudzwa kwe nucleotide analogues yekuisa yakatarisana neC uye T zvichiratidzwa nekudzikira kwehukama hwekuisa effice nekudzikira kwehuwandu hweWatson-Crick H-bond uye kuwedzera nhamba yevanopa-vanopa uye vanogamuchira-anogamuchira kusangana.Kusarudzwa kwekuisa nucleotide kwakakura kutarisana ne5'-T pane 3'-T yethymine dimer, maererano nebasa rekare rinoratidza kuti 5'-T inobatwa zvakasimba kudarika 3'-T.Uyezve, kuiswa kweA yakatarisana neTs yese ye dimer inoita seyakayananiswa neWatson-Crick base pairing uye kwete neHoogsteen base pairing zvichibva pane zvakada kufanana zvekuisa mashandiro eA uye 7-deaza-A, iyo yekupedzisira iyo isina H- bonding kugona paN7.Iko kushanda kwakanaka kwekuiswa kwema nucleotides anogona kuumba Watson-Crick base pairs anoenderana neaya eKlenow fragment, nepo Klenow fragment ichinyanya kusarura kusawirirana, zvichienderana nehukuru hwayo kusarudza.Mhedzisiro iyi inosimbisa kukosha kweH-bonding neWatson-Crick base pair geometry mukusarudzwa kwemanucleotides nepol eta neKlen ow fragment, uye basa diki rekusarudzwa kwechimiro mukuiswa nepol eta nekuda kwekuvhurika kwayo uye kushoma. inomanikidzirwa inoshanda saiti.