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Kunyatso ongorora akati wandei metabolites e5-fluorouracil (5-FU) uye maviri endogenous monophosphate nucleotides, takagadzira nzira yekutanga yakavakirwa pamvura chromatography-tandem mass spectrometry (LC-MS/MS).Iyi bvunzo yakabvumira kutsunga kwe: (i) intracellular kugadzirwa kwe5-fluoro-2'-deoxyuridine-5'-monophosphate (5-FdUMP) kubva ku5-FU kana 5-fluoro-2'-deoxyuridine (5-FdUrd) , (ii) kukanganisa kwe5-FdUMP concentration pane intracellular 2′-deoxyuridine-5′-monophosphate (dUMP)/thymidine-5′-monophosphate (TMP) ratio, uye (iii) secretion extent ye5-FdUMP uye 5-FU kubva kumasero akagadzirwa nevanhu neABC vatakuri.Pasi pemamiriro edu ekuedza, maseru akaiswa ne5-FU kana 5-FUrd.Zvadaro, mapuroteni emasero akaiswa nemethanol.Iyi nzira yakapa kudzoreredza kwakanyanya.Uye zvakare, kuyera 5-FU uye 5-FdUMP secretion kubva kumaseru, takaita quantification yemamorekuru aya mutsika yepakati.Midhiya yakaitwa jekiseni zvakananga (5-FU) kana pasi yakaenda yakasimba chikamu kudhirowa uchishandisa Oasis Wax yekubvisa katuriji (5-FdUMP).Kupatsanurwa kweanoongorora kwakaitwa padC18 Atlantis 3.5 μm, (100 mm × 2.1 mm id) ine isocratic mode uchishandisa ammonium formate buffer/methanol/mvura (5/5/90, v/v) sechikamu chenhare.Nguva yekumhanya haina kudarika 6.2 min.Iwo ma analytes aive ionized mune electrospray interface pasi peiyo negative ion mode.Takasimbisa nzira pamusoro pehuwandu hwe2.5-150 ng mL -1 maererano nemishonga.Intra- uye inter-assay musiyano wakaderera pane 10% pamusoro pemazuva manomwe.Ese makomisheni aive akatsiga mumaseru kana mutsika yepakati apo masampuli akachengetwa pa -20 ° C kweinenge mavhiki maviri, uye mushure mekutenderera katatu kwechando.Hapana matrix maitiro akaonekwa mune ese midhiya.