Products

APS-5 inonzi chemiluminescent substrate inobva ku9,10-dihydroacridine, iyo inonyanya kushandiswa ELISA kuonekwa kwemakemikari ane alkaline phosphatase AP conjugates;9,10-dihydroacridine muchimiro cheAPS-5 Chimiro che hydroacridine chinogona kubudisa chiedza chinoramba, chakagadzikana uye chinobudirira pasi pechiito che AP, uye APS-5 inonyatsokodzera phosphatase basa rekugadzirisa assay uye phosphatase enzyme-yakabatana immunoassay.

Seye luminescent probe, inoshandiswa mukudzidza kwemajini machipisi.Maitiro acho akanyorwa ne acridan (9,10-dihydroacridine) se substrate uye alkaline phosphatase, ichigadzira yakawedzera-yakasimba chemiluminescence.Inopa hukuru hwekunzwa uye nyore kwekushandisa alkaline phosphatase conjugation panguva yekuongorora chemiluminescent.Alkaline phosphatase inowanikwa mune isingasviki 10-19 mol, inokwira nekukurumidza kuderedza nguva yekuona uye kuwedzera kubuda, uye kutsetseka kweiyo linear calibration curve inorongwa ne logarithmic yakaenzana ne1.0.Imwe yakawanda kana yakawanda ye enzyme inogadzira imwe yakawanda kana yakawanda ye luminescence, inoenderera mberi luminescence-isingade zvakanyanya panguva yekuedzwa.Luminescence intensity inogona kuverengwa kubva payakagadzirwa linear calibration curve chero nguva, uye mhedzisiro yekuongorora haina hanya netembiricha iri pakati pe22 ° C - 35 ° C, ichidzikisa kurongeka kunodiwa kudzora tembiricha.

mukana:

1 High senitivity - maprotein markers pasi pe5pg anogona kuonekwa;

2 High luminous intensity - iyo inopenya peak inogona kusvika munguva pfupi;

3 Inoenderera uye yakagadzikana luminescence - iyo luminescence process haina kukanganiswa nekupisa mukati me25-35 ℃, uye haidi tembiricha kutonga.

Kuenzanisa kwekushandiswa kweanowanzo shandiswa luminescent substrates APS-5 (193884-53-6) uye AMPPD (122341-56-4)

Ose APS-5 (193884-53-6) uye AMPPD (122341-56-4) machemiluminescent substrates yealkaline phosphatase, uye ese ari maviri anonzi ekupedzisira-akakwira senitivity yekuona reagents, saka aya mareagents ari mukuona Izvo zviri nani uye. chii chakaipa?Ungasarudza sei kana uchiishandisa?

Kune rimwe divi, kana APS-5 ichishandiswa sealkaline phosphatase yekuona substrate, iyo yekuona senitivity inogona kusvika 10-19 molar concentration, nguva yekukura kwemavara inogona kusvika pakakwirira pepamusoro mumasekonzi gumi, uye fluorescence inogara kwenguva refu. nguva.Munzira idzi, APS 5 inogona kunzi ine zvivakwa zvakanakisa.Nekudaro, APS-5 inogumira mukupisa kwekushandisa, uye tembiricha yayo inopa ruvara ingave iri pasi pe20'C.Kune rumwe rutivi, apo AMPPD inoshandiswa se substrate yekucherechedza ye alkaline phosphatase, kunzwisiswa kwekuona kunonziwo ultra-high senitivity, asi haigoni kusvika pamwero we APS 5. Nguva yekukura kwemavara inosvika pakukwirira kwepamusoro pamasekonzi anenge makumi matatu. , uye nguva yefluorescence yakareba.AMPPD yakanakisa muzvivakwa zviri pamusoro, asi yakaderera kune APS5, asi tembiricha yayo yekupa ruvara haina kuomarara seAPS5, uye tembiricha yekuchengetera ye2--8C iri nyore pane APS-5.Muchidimbu, kunyangwe APS-5 ichipfuura AMPPD mukuonekwa kwayo, inorasikirwa mukugadzikana.Nzira yekusarudza nayo zvinoenderana nezvinodiwa zvekuonekwa.

1. Nhanganyaya yeAPS-5 (193884-53-6):

Zita reChirungu: APS-5

Nhamba yeCAS: 193884-53-6

Molecular formula: C21H15ClNO4PS Na2

Molecular uremu: 489.819

Kuonekwa: off-white solid powder

Zviri mukati: ≥98%

Zvinodiwa: 1g, 5g, 10g

Kuchengetedza mamiriro: -20 ℃ kuchengetedza

(4-Chlorophenylmercapto) (10-methyl-9,10-dihydroacridine methylene) phosphate disodium munyu, inonzi APS-5, inonzi chemiluminescent reagent uye inoshandiswa sechemiluminescent substrate.Inopa hukuru hwekunzwa uye nyore kwekushandisa alkaline phosphatase conjugation panguva yekuongorora chemiluminescent.Maitiro acho akanyorwa ne acridan (9,10-dihydroacridine) se substrate uye alkaline phosphatase, ichigadzira yakawedzera-yakasimba chemiluminescence.APS-5 ndiyo yakanaka reagent yekugadzika kwephosphatase chiitiko nemhinduro assays uye phosphatase enzyme-yakabatana immunoassays.Seye luminescent substrate, APS-5 ine yakakwirira yekuona senitivity uye pfupi yekuita nguva.

2. Yakadzama sumo yeAMPPD (122341-56-4)

Zita reChirungu: AMPPD

Nhamba yeCAS: 122341-56-4

Molecular formula: C18H23O7P

Molecular uremu: 382.344781

Kuonekwa: chena poda

Kuchena: ≥97%

Zvinodiwa: 1g, 5g, 10g

Kuchengetedza mamiriro: -20 ℃ kuchengetedza

Chengetedzo: Inofanira kunge yakaoma kuti irege kuparara kazhinji uye lyophilization