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1.Kugadziriswa kwebasa rema enzymes ane chokuita nekabhohydrate metabolism inokosha kune mbatata inotonhora-induced sweetening (CIS).Inoveli RING chigunwe gene SbRFP1 yakaumbwa uye kutaura kwayo kwakawanikwa kuchitonhora-inducible mumatato tubers eCIS-resistant genotypes.Shanduko yeSbRFP1 mumatato yakasimbisa basa rayo mukudzivisa β-amylase uye invertase chiitiko, izvo zvakazokonzeresa kudzora starch uye sucrose kudzikira uye kuunganidzwa kwekudzikisa shuga mumachubhu akachengetwa anotonhora.Izvi zvakawanikwa zvinoratidza zvakasimba kuti SbRFP1 inogona kushanda senge isina kunaka regulator yeBAM1 uye StvacINV1 kudzikamisa kuunganidzwa kwekudzikisa shuga mukuita kwembatatisi CIS.

2.Chinangwa chebasa iri chaive chekudzidza pesvedzero yekubika kubika pa pectin methylesterase (PME) uye endogenous α- uye β-amylase mabasa mumhando dzakasiyana (cortex uye pith) yembatatisi yakasvibirira uye inopisa cv.Agria.Matembiricha matatu akasiyana ekubika akasarudzwa (55, 70 uye 85 °C).Payedzero yega yega yekubika, maprofiles enguva-yetembiricha akarekodhwa uye dhigirii rekubika rakaratidzwa maererano nekubika chinhu.Kubika kweSteam kwakabatsira zvakanyanya kumisa PME pa55 ° C uye kudzikisa chiitiko chayo patembiricha yekupedzisira (85 ° C) , ine huwandu hwepamusoro mucortex (0.3745 ± 0.0007 µmol galacturonic acid (GA) g(-1) uremu hutsva (FW) min(-1) ) zvichienzaniswa nepith (0.2617 ± 0.0012 µmol GA g(-1) FW min(-1) ).Kuvapo kwekupisa-labile uye kupisa-yakagadzika isoforms yePME mune inofungidzirwa mbatatisi tishu yaifungidzirwa zvakare.Kurapwa kwekupisa neutsi kwakakonzera kudzikira kukuru kweapo- uye β-amylase zviitiko mumatishu ese ari maviri zvichienzaniswa nembatatisi mbishi kusvika, kunyangwe pasina kudzima zvachose.Starch-degrading enzymes akawanikwa zvakare akagovaniswa zvakasiyana mune mbishi tuber.Steam yekubika yakakanganiswa nenzira dzakasiyana-siyana yakaongororwa yasara enzymatic chiitiko mumatishu anoonekwa embatatisi cv.Agria.Imwe tsvakiridzo inodiwa kusimbisa zvawanikwa.