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Adenosine is endogenous regulator yevascular tone.Ichi chiitiko cheadenosine chinopedzwa nekutora kwayo uye metabolism ne microvascular endothelial cells (MVEC).Mutakuri mukuru anobatanidzwa ndeye ENT1 (equilibrative nucleoside transporter subtype 1).MVEC inotaurawo iyo nucleobase transporter (ENBT1) iyo inobatanidzwa mune cellular flux yeadenosine metabolites yakadai se hypoxanthine.Shanduko mune chero yeaya masisitimu ekufambisa angakanganisa bioactivity yeadenosine uye metabolism yayo, kusanganisira kuumbwa kweokisijeni isina radicals.MVEC yakaparadzaniswa kubva kune skeletal tsandanyama yeENT1 (+/+) uye ENT1 (-/-) mbeva dzakaiswa pasi pekunetseka kweoxidative kunokonzerwa nesimulated ischemia/reperfusion kana menadione.Mabasa eENT1 uye ENBT1 akaongororwa zvichienderana ne zero-trans influx kinetics ye radiolabeled substrates.Paive nekudzikiswa kwechiyero cheENBT1-mediated hypoxanthine uptake neENT1(+/+) MVEC yakarapwa menadione kana mushure mekusangana nemamiriro anotevedzera isc hemia/reperfusion.Muzviitiko zvese izvi, iyo superoxide dismutase mimetic MnTMPyP yakadzora kurasikirwa kweENBT1 chiitiko, ichibatanidza superoxide radicals mumhinduro.Kusiyana neizvi, MVEC yakaparadzana neENT1(-/-) mbeva yakaratidza kusadzikiswa kwekuita kweENBT1 pakurapwa nemenadione kana kuti simulated ischemia/reperfusion, asi vaive nedanho rakanyanya repamusoro recatalase chiitiko maererano neENT1(+/+) MVEC.Iyi data inoratidza kuti ENBT1 basa rakaderera muMVEC mukupindura kune yakawedzera superoxide radical iyo inobatanidza ne ischemia / reperfusion kukuvara.MVEC yakaparadzaniswa kubva kuENT1 (-/-) mbeva hairatidze kudzikiswa uku muENBT1, pamwe nekuda kwekuwedzera kwekuita kwecatalase.