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Infrared spectroscopy yakashandiswa kumepu substrate-protein interactions: conformational shanduko ye sarcoplasmic reticulum Ca (2+) -ATPase pa nucleotide binding uye ATPase phosphorylation yakaongororwa pachishandiswa substrate ATP uye ATP analogues (2'-deoxy-ATP, 3). '-deoxy-ATP, uye inosine 5'-triphosphate), iyo yakagadziridzwa pamapoka chaiwo anoshanda e substrate.Kugadziriswa ku2'-OH, iyo 3'-OH, uye boka reamino readenine rinoderedza chiyero chekusunga-induced conformational shanduko yeATPase, pamwe nemigumisiro yakasimba yakaonekwa kune maviri ekupedzisira.Izvi zvinoratidza kunzwisiswa kwemaitiro eiyo nucleotide-ATPase yakaoma kune yega kupindirana pakati penucleotide neATPase.Mapoka ese akadzidzwa akakosha pakusunga uye kupindirana kweboka rakapihwa ligand neATPase zvinoenderana nekudyidzana kwemamwe mapoka e ligand.Phosphorylation yeATPase yakacherechedzwa yeITP uye 2'-deoxy-ATP, asi kwete ye3'-deoxy-ATP.Hapana hukama hwakananga pakati pehupamhi hwekuchinja kwekugadzirisa pane nucleotide kusunga uye chiyero che phosphorylation chinoratidza kuti huwandu hwakazara hweATP-induced conformational shanduko haifaniri kumanikidzirwa kune phosphorylation.Sekucherechedzwa kweiyo nucleotide-ATPase yakaoma, kuumbwa kwekutanga phosphorylated ATPase yepakati E1PCa (2) kunoenderanawo neiyo nucleotide, zvichiratidza kuti ATPase nyika dzine yunifomu shoma pane yaimbotarisirwa.